常用质粒载体中-报告基因，看家基因序列

黄帮主 · 发表于 2023-11-6 16:29:19

本帖最后由黄帮主于 2023-11-6 16:54 编辑

Nagai T, et al. (2002) A variant of yellow fluorescent protein with fast and efficientmaturation for cell-biological applications. Nat Biotechnol 20:87–90.
eYFP 239aa
黄色荧光蛋白，在GFP的基础上进行点突变，促进蛋白有效快速成熟。
MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

黄帮主 · 发表于 2023-11-6 23:25:46

增强型的蓝色荧光蛋白：cerulean
184个aa
ldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlvttltwgvqcfarypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnrielkgidfkedgnilghkleynaisdnvyitadkqkngikanfkirhniedgsvqladhyqqntpigdgpvllpdnhyl

黄帮主 · 发表于 2024-9-25 14:40:16

也是DNA连接酶，来自短尾噬菌体
MMNIKTSPFKAVSFVESAVKKALDNAGYLIVDCKYDGVRGNICVDNTANSCWLSRVSKTIPALEHLNGFDVRWKRLLNDDRCFYKDGFMLDGELMVKGVDFNTGSGLLRTKWTDTKNQEFHEELFVEPVRKKDKVPFKLHTGHLHVKLYAILPLYIVESGEDCDVMTLLMQEHVKNMLPLLQEYFPEIEWQVAESYEVYDMVELQQLYEKKREEGHEGLIVKDPMCIYKRGKKSGWFKMKPENEADGIIQGVNWGTEGLANEGKVIGFSVLLESGCVVNANNISRELMDEFTANVKEHGEDYYNGWACQISFMEETPNGNLRHPSFTCFRGTEDNPQEKM

黄帮主 · 发表于 2024-9-25 14:44:20

RNA聚合酶抑制剂：
MSNANTGSLSVDNKKFWATVESSKHSFEVPIYAETLDEAVELAEWQYVPAGFEVTRVRPCVAPK

黄帮主 · 发表于 2024-9-25 21:24:58

RNA聚合酶：
MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVNLHRQNAGVVGQDSETIELTPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREELPMKPEDIDTNPEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEDNHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLLDEVGGLAVNLLPSATVQDIYGIVAKKVNVILQADVINGTDNEVVTVTDENTGEIPEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWEAVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLQDILKSDFAFA

黄帮主 · 发表于 2024-10-5 10:35:10

溶菌酶氨基酸序列，来自K-12菌株MG1655：MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKDIVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ

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