Continuous cultures of fused cells secreting antibody of predefined specificity

傲慢与偏见

The hybrid Sp-1 gave direct plaques and this suggested that it produces an lgM antibody. 杂交瘤细胞Sp-1直接空斑实验显示细胞产生IgM抗体。

傲慢与偏见

This is confirmed in Fig. 4 which shows the inhibition of SRBC lysis by a specific anti-lgM antibody. IEF techniques usually do not reveal 19S IgM molecules. IgM is therefore unlikely to be present in the
unreduced sample a (Fig. 3B) but u chains should contribute to the pattern obtained after reduction (sample a, Fig. 3A).


The above results show that cell fusion techniques are a
powerful tool to produce specific antibody directed against a
predetermined antigen. lt further shows that it is possible to
isolate hybrid lines producing different antibodies directed
against the same antigen and carrying different effector functions
(direct and indirect plaque).
The uncloned population of P3-spleen hybrid cells seems
quite heterogeneous. Using suitable detection procedures it
should be possible to isolate tissue culture cell lines making
different classes of antibody. To facilitate our studies we have
used a myeloma parental line which itself produced an lg.
Variants in which one of the parental chains is no longer expressed
seem fairly common in the case of Pl-P3 hybrids
(Fig. lh). Therefore selection oflines in which only the specific
antibody chains are expressed seems reasonably simple.
Alternatively, non-producing variants ...,f myeloma lines could
be used for fusion.

傲慢与偏见

We used SRBC as antigen. Three different fusion experiments were successful in producing a large number of antibody producing cells. Three weeks after the initial fusion, 33/1,086 clones (3 %) were positive by the direct plaque assay. The cloning efficiency in the experiment was 50~'~- In another experiment, however, the proportion of positive clones was considerably lower (about 0.2 %). In a third experiment the hybrid population was studied by limiting dilution analysis. From 157 independent hybrids, as many as I 5 had anti-SRBC activity. The proportion of positive over negative clones is remarkably high. It is possible that spleen cells which have been triggered during immunisation are particularly successful in giving rise to viable hybrids. It remains to be seen whether similar results can be obtained using other antigenes.

傲慢与偏见

The cells used in this study are all of BALB/c origin and the hybrid clones can be injected into BALB/c mice to produce solid tumours and serum having anti-SRBC activity. It is possible to hybridise antibody-producing cells from different origins。 Such cells can be grown in vitro in massive cultures to provide specific antibody. Such cultures could be valuable for medical and industrial use .
本研究中所有到的细胞均来自BALB/c小鼠，融合后的杂交瘤细胞可以重新注射Balb/c小鼠产生实体瘤，血清具有抗-SRBC活性。这种融合的产生抗体的杂交瘤细胞也可能有其他来源，融合成功后能在体外进行大规模培养产生特异性抗体，用于医学和工业生产。